Protocols
Bead protocols
| Preparation of Protein-A - anti-DIG-coated latex beads | 2024.04.28. |
| Preparation of Protein-A-antibody-coated latex beads | 2024.04.28. |
Bead-tailed Actin Filaments  |
2024.05.01. |
| Preparation of Ni-NTA beads |
2024.05.01. |
| Preparation of photoreactive beads | 2024.04.28. |
Electrophoresis
| Gradient Gels for titin | 2024.05.01. |
| Ammoniacal Silver Staining of Electrophoretic gels | 2024.05.02. |
Fluorescent labeling
| Fluorescent labeling of actin with Rhodamine-phalloidin | 2024.04.28. |
| Citoszkeleton rodamin-falloidin festése (in Hungarian) | 2024.04.28. |
Other
| Cleaning of coverslips for Total Internal Fluorescence Microscopy | 2024.04.28. |
| DNA end biotinylation for optical tweezers experiment | 2024.04.28. |
| Actomyosin in vitro motility assay | 2024.04.28. |
| Biokémiai laboratórium oldatai (in Hungarian) | 2024.05.02. |
Protein preparation
| Purification of muscle actin I: Preparation of acetone powder | 2024.04.28. |
| Isolation of actin from acetone powder | 2024.04.28. |
| Preparation of native titin from rabbit longissimus dorsi | 2024.04.28. |
| Preparation of myofibrils from glycerinated psoas muscle | 2024.04.28. |
| Myomesin Preparation | 2024.04.28. |
| Preparation of myosin | 2024.04.28. |
| Preparation of Troponin-Tropomyosin (Native tropomyosin) and Reconstitution of Thin Filament (Regulated Actin) | 2024.04.28. |
| Papain Mg-S1 preparation | 2024.04.28. |
| HMM preparation | 2024.04.28. |
| Preparation of and in vitro motility assay on human cardiac myosin | 2024.04.28. |
| Preparation of desmin | 2024.04.28. |
| Preparation of glycerinated psoas muscle | 2024.04.28. |
| Össz hiszton preparálás borjú timuszból (in Hungarian) | 2024.04.28. |